![]() ![]() Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow. ![]() The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Note, the concentration units are unspecified use whichever units are applicable for your standards (the calculated concentrations will use these same unspecified units). The concentrations of the samples are determined from the fit with any specified dilution factors applied. measurement) are plotted on semi-log axes and a 4PL is made through the points. These not only show you how to use Prism, but also review the principles of nonlinear regression, including comparing models, identifying outliers, global fitting, and more. ![]() If you are new to Prism, choose from the sample XY data sets. The standard data points (concentration vs. Prism makes it quite easy to fit a model to your data. If a blank group is included on your layout, the mean of the blank replicates is first subtracted from the raw data measurements (the corrected values are then used in the fit). This analysis optionally includes a background correction step. This method is widely used and cited in data analysis for typical ELISAs. Four Parameter Logistic (4PL) curve fit suitable for calculating concentrations from symmetrical sigmoidal calibrators.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |